The actor, 66, looked uncharacteristically frustrated as he jabbed a finger at the younger man while attending the event alongside his wife Rita Wilson, also 66.
Actress and producer Rita, who also stars in the film, could be seen holding her hand up in front of her as she spoke to the guest.
Dressed in a smart tuxedo suit and wearing a bow tie, he could be seen speaking to the pair in what appeared to be an attempt to calm the situation.
However, Tom seemed to remain miffed as the trio spoke before heading down the red carpet to pose for pictures at the event.
Miffed: Tom Hanks, 66, appeared to be embroiled in a heated discussion as he walked the red carpet at the premiere for his film Asteroid City during the Cannes Film Festival on Tuesday
Irritated? The actor could be seen jabbing his finger out in front of him while his brow was very furrowed as he stood alongside his wife Rita Wilson, also 66
Cooling off: The guest could be seen speaking to the pair as he appeared to be attempting to calm the situation
MailOnline has contacted a representative for Tom for comment.
Tom cut a dapper figure as he wore a black tuxedo and matching bow tie which he teamed with a smart white shirt for the premiere.
His partner Rita looked glamorous in a chic black dress with a a long black cloak which trailed the ground behind her.
The garment was adorned with silver jewels which shimmered under the spotlights on the red carpet.
Both Tom and Rita appeared in good spirits as they posed for pictures before heading inside to watch the film.
The movie, which is a coming-of-age movie about stargazing in the desert, is directed by quirky filmmaker Wes Anderson.
Asteroid City is set in the 1950s in a fictional American desert town that has an eerie feel to it.
At a Junior Stargazer/Space Cadet convention, which is designed to unite students and parents in scholarly competition, things go haywire when a world event takes place.
Heated? Actress and producer Rita could be seen holding her hand up in front of her as she spoke to the guest
Looking good: Tom cut a dapper figure as he wore a black tuxedo and matching bow tie which he teamed with a smart white shirt for the premiere
Glitterati: His partner Rita looked glamorous in a chic black dress with a a long black cloak which trailed the ground behind her
The story centres on a widower played by Jason Schwartzman who takes his four children to see their grandfather, played by Hanks.
‘In my loneliness, I’ve learned to give complete and unquestioning faith to the people I love,’ Hanks’ character tells Schwartzman’s. ‘I don’t know if that includes you, but it included my daughter and your four children.’
Their car breaks down so they take it to Asteroid City to get repaired.
His son, played by Jake Ryan, is fascinated by the city’s annual junior stargazer competition.
Soon the family becomes drawn in by the strange city where there are fears about aliens.
Hanks time: Asteroid City is set in the 1950s in a fictional American desert town that has an eerie feel to it (Tom, right, is pictured with Jason Schwartzman in the film)
Also in the movie is Tom’s wife Rita Wilson as well as Tilda Swinton, Matt Dillon, Bryan Cranston, Ed Norton, Adrien Brody and Liev Schreiber help round out the cast.
Hope Davis, Stephen Park, Rupert Friend make appearances as well.
And Uma Thurman and Ethan Hawke’s daughter Maya Hawke has a role in the offbeat movie.
Steve Carell, Willem Dafoe, Tony Revolori, Grace Edwards, Aristou Meehan, Sophia Lillis, Ethan Lee and Jeff Goldblum are included as well.